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Bulletin of the Chemists and Technologists of Bosnia and Herzegovina
Issue 62
Publication date: June 2024 |
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Table of contents
Evaluation of Antioxidant, Antibacterial and Cytotoxic Effects of Pink Pepper Fruit Essential Oil Original Scientific Article
Horozić, E., Ademović, Z., Dautović, E., Kolarević, L., Cilović Kozarević, E., Karić, E., Ibišević, M., Huseinović, E., Husejnagić, D.
DOI: 10.35666/2232-7266.2024.62.01
UDK: 547.913:582.672
Abstract: Pink pepper is classified into the Californian/Peruvian type (Schinus molle) and the Brazilian type (S. terebinthifolia). According to studies, pink pepper extracts and essential oils have shown anti-inflammatory, antioxidant, genotoxic and antidiabetic properties in in vitro and in vivo studies. The results of bioactivity tests vary depending on the geographical origin of the pepper. In this paper, the cytotoxic, antioxidant and anti-bacterial effects of the essential oil of the commercial pink pepper fruit from the Tuzla market were investigated. To assess the cytotoxic potential, a tetrazolium salt reduction (MTT) viability assay was performed. The antioxidant potential was examined spectrophotometrically, using DPPH and FRAP methods. Diffusion techniques were used to evaluate the antibacterial activity of the essential oil. Using GC/MS, 24 components of red pepper essential oil were identified, of which α-pinene, α-phellandrene, δ-3-carene and D-limonene dominate. The studied pink pepper essential oil inhibited the cell proliferation in the HeLa cell line, causing a dose-dependent cytotoxic effect (IC50=389.46 µg/mL). The essential oil inhibits DPPH radicals. The reducing ability is relatively weak. For the essential oil, an extremely good ability to inhibit the growth of the bacteria used in this study was confirmed. These results indicate a very high potential of essential oil of pink pepper fruit as an inhibitor of pathogenic organisms.
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 62, 01-06
The inhibitory properties of the boiling extracts from Malus sylvestris andSyringa vulgaris flowers on the corrosion of stainless steel in sulphuric acid medium Original Scientific Article
Oo Z. Y. M., Nikolaychuk P. A.
DOI: 10.35666/2232-7266.2024.62.02
Abstract: The inhibitory properties of the Malus sylvestris flower and Syringa vulgaris flower boiling extracts against the stainless steel EN Fe37-3FN corrosion in 0.5 M sulphuric acid medium were studied using electrochemical methods, including open circuit potential measurement, potentiodynamic polarisation and EIS. The addition of 10 mg/l of the Malus sylvestris flower extract slows the corrosion by 15%, and that of of 10 g/l – by 65%, while the addition of 10 mg/l of the Syringa vulgaris flower extract slows the corrosion by 30%, and that of of 1 g/l and more – by 65%. The Langmuir absorption model describes the adsorption of the components of the extracts on a surface of the steel, and the adsorption is physical in its nature. The Malus sylvestris flower and Syringa vulgaris flower extracts reveal themselves as interesting and environmentally safe substances for the steel corrosion rate reduction in acidic environments.
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 62, 07-12
Binding constants and in silico analysis of albumin interaction with phenolic acids and flavonoids Original Scientific Article
Kristić, D., Dizdar, M., Ćavar Zeljković, S., Kopečny, D., Topčagić, A.
DOI: 10.35666/2232-7266.2024.62.03
UDK: 547.962.3:547.588.3
Abstract: : In this study, fluorescence techniques were utilized to investigate the interactions of selected phenolic acids (PAs) and flavonoids (FLs) with bovine serum albumin (BSA) under physiological conditions. The binding of PAs/FLs with BSA was investigated at three temperatures: 292, 303 and 310 K. From the obtained spectra, the Stern-Volmer constant (Ksv), bimolecular quenching constant (kq), binding constants (Kb), and binding site number (n) constants were calculated. Presented results indicate that fluorescence quenching of BSA in the presence of phenolic acids/flavonoids is a static quenching process. The strongest static binding occurs during the formation of the BSA-pHBA (p-hydroxybenzoic acid) complex (kq = 57.1×1012 M-1 s-1 at 292 K), and BSA-Que (quercetin) complex (kq = 42.8×1012 M-1 s-1 at 292 K). The structure of PAs/FLs was revealed to significantly affect the binding/quenching process and additionaly, fluorescence resonance energy transfer studies confirmed the static nature of this process. The results of synchronous fluorescence spectra suggest changes in the microenvironment of tyrosine. Three-dimensional spectra showed changes related to the backbone structures of the protein chain (caused by the π-π∗transition of the carbonyl group). Furthermore, thermal denaturation was performed by nano differential scanning fluorimetry (nanoDSF) and transition temperature (Tm) values for BSA complexes with PAs/FLs are slightly lower than Tm for BSA, except Tm for BSA complexes with kaempferol and chrysine. According to in silico analysis, theoretically, caffeic acid and quercetin showed the best binding position with albumin (4F5S).
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 62, 13-24
Application of hydrophobic solvents based on L-menthol, as greener alternatives to classical solvents for Pb(II) ions extraction Original Scientific Article
Suljkanović, M., Suljagić, J., Bjelić, E, Mandžukić, A.
DOI: 10.35666/2232-7266.2024.62.04
UDK: 547[544.722.132:541.8]
Abstract: Deep eutectic solvents (DESs) as a new-generation of solvents are receiving increasing attention as environmentally friendly solvents in various analytical techniques. These solvents are new generation solvents, and based upon what they are derived from, they can be the safest, cheapest, and most effective extraction methods available. With DES, the extraction efficiency and metal ion recycling are significantly improved. In this work, the potential application of these solvents for the extraction of Pb(II) ions was investigated. For that purpose, hydrophobic DESs (HDESs), based on L-menthol as H-acceptor and decanoic acid as H-donor, were prepared at molar ratios of acceptor to donor of 1:1 and 1:2. In the optimized extraction procedure, the Pb(II) ions were extracted into the organic phase with the efficiency of 94.3% and 97.3% for 1:1Men:DecA and 1:2 Men: Dec A, respectively. The results also showed that unlike of classical liquid-liquid extraction methods, in the original solutions, counterions are not required to transfer the analyte to the hydrophobic phase. Furthermore, no ligands were required to transfer the analyte ions to the HDES phase: the results show that the extraction efficiency of 1:1 HDES decreased to 88.65% in the presence of 18C6, and to 96.5% for 1:2 HDES. Comparable results for HDES (1:1 Men: DecA) extraction efficiency in the proposed method with the efficiency of 1,2-dichloroethane and chloroform in classical methods (96.7% and 92%, respectively), without counterions and without the requirement for ligands as carriers, make this HDES-based extraction method simpler, less expensive, and most importantly, more environmentally friendly.
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 62, 25-30
The relationship between the physical and lipid characteristics of eggs from hens that were fed a diet supplemented with fat Original Scientific Article
Ohran, H., Hodžić, A., Semić, A., Ajanović, A., Crnkić, Ć.
DOI: 10.35666/2232-7266.2024.62.05
UDK: 577.1:637.41
Abstract: The aim of this study was to investigate the potential correlations between certain physical and lipid characteristics of eggs from hens fed diets supplemented with three different types of fat. A six-week-last experiment was conducted on 45 Brown Lohman laying hens, 56 weeks of age. Animals were randomly divided into three groups of 15, and fed one of three experimental diets supplemented with either 3% of fish oil, palm olein, and lard. Samples of 10 eggs per group were collected at the start and at the end of the experiment to determine four physical and six lipid characteristics in boiled eggs. The supplementation of the hens' diet with fat at significantly affected the physical characteristics of the The biggest impact on such finding for total sample of investigated eggs had palm olein as a supplement in hen diet. Among the three investigated fat supplements, the addition of 3% lard to the laying hen diet resulted in the smallest total number of significant correlations between investigated physical and lipid traits. Comparing the end to the start of our experiment, supplementation of hen diet with fat decreases the number of correlations among egg physical and lipid characteristics, what can be considered as a positive result from both egg producer and consumer interests.
Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 62, 31-38